Chitosan-grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible enzyme immobilization


Arica M. Y. , YILMAZ M. , Bayramoglu G.

JOURNAL OF APPLIED POLYMER SCIENCE, vol.103, no.5, pp.3084-3093, 2007 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 103 Issue: 5
  • Publication Date: 2007
  • Doi Number: 10.1002/app.25466
  • Journal Name: JOURNAL OF APPLIED POLYMER SCIENCE
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.3084-3093
  • Keywords: membrane, contact angle, immobilized enzyme, adsorption, glucose oxidase, GLUCOSE-OXIDASE, CANDIDA-RUGOSA, ADSORPTION, COMPONENTS, STABILITY, BEHAVIOR, INVERTASE, CATALASE, LIPASE, ENERGY

Abstract

Epoxy group-contain g poly(hydroxyethyl methacrylate/glycidyl methacrylate), p(HEMA/GMA), membrane was prepared by UV initiated photopolymerization. The membrane was grafted with chitosan (CH) and some of them were chelated with Fe(III) ions. The CH grafted, p(HEMA/GMA), and Fe(III) ions incorporated p(HEMA/ GMA)-CH-Fe(III) membranes were used for glucose oxidase (GOD) immobilization via adsorption. The maximum enzyme immobilization capacity of the p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes were 0.89 and 1.36 mg/mL, respectively. The optimal PH value for the immobilized GOD preparations is found to have shifted 0.5 units to more acidic PH 5.0. Optimum temperature for both immobilized preparations was 10 degrees C higher than that of the free enzyme and was significantly broader at higher temperatures. The apparent K-m values were found to be 6.9 and 5.8 mM for the adsorbed GOD on p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes, respectively. In addition, all the membranes surfaces were characterized by contact angle measurements. (c) 2006 Wiley Periodicals, Inc.