Electrochemical biosensing of galactose based on carbon materials: graphene versus multi-walled carbon nanotubes

DALKIRAN B., ERDEN P. E., Kilic E.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, cilt.408, sa.16, ss.4329-4339, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 408 Sayı: 16
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s00216-016-9532-x
  • Dergi Adı: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4329-4339
  • Anahtar Kelimeler: Amperometry, Co3O4 nanoparticles, Galactose biosensor, Graphene, Multi-walled carbon nanotubes, AMPEROMETRIC BIOSENSOR, HYDROGEN-PEROXIDE, ENZYME ELECTRODE, OXIDASE, GLUCOSE, MONOSACCHARIDES, CHROMATOGRAPHY, NANOPARTICLES, DISACCHARIDES, POLYPYRROLE
  • Ankara Hacı Bayram Veli Üniversitesi Adresli: Evet

Özet

In this study, two enzyme electrodes based on graphene (GR), Co3O4 nanoparticles and chitosan (CS) or multi-walled carbon nanotubes (MWCNTs), Co3O4 nanoparticles, and CS, were fabricated as novel biosensing platforms for galactose determination, and their performances were compared. Galactose oxidase (GaOx) was immobilized onto the electrode surfaces by crosslinking with glutaraldehyde. Optimum working conditions of the biosensors were investigated and the analytical performance of the biosensors was compared with respect to detection limit, linearity, repeatability, and stability. The MWCNTs-based galactose biosensor provided about 1.6-fold higher sensitivity than its graphene counterpart. Moreover, the linear working range and detection limit of the MWCNTs-based galactose biosensor was superior to the graphene-modified biosensor. The successful application of the purposed biosensors for galactose biosensing in human serum samples was also investigated.