In this study, a new amperometric carbon paste enzyme electrode for determination of ethanol was developed. The carbon paste was prepared by mixing alcohol dehydrogenase, its coenzyme nicotinamide adenine dinucleotide (oxidized form, NAD(+)), poly(vinylferrocene) (PVF) that was used as a mediator, graphite powder and paraffin oil, then the paste was placed into cavity of a glass electrode body. Determination of ethanol was performed by oxidation of nicotinamide adenine dinucleotide (reduced form, NADH) generated enzymatically at +0.7 V. The effects of enzyme, coenzyme and PVF amounts; pH; buffer concentration and temperature were investigated. The linear working range of the enzyme electrode was 4.0 x 10(-4) - 4.5 x 10(-3) M, determination limit was 3.9 x 10(-4) M and response time was 50s. The optimum pH, buffer concentration, temperature, and amounts of enzyme, NAD(+) and PVF for enzyme electrode were found to be 8.5, 0.10 M, 37 degrees C, 2.0, 6.0, and 12.0 mg, respectively. The storage stability of enzyme electrode at +4 degrees C was 7 days. Enzyme electrode was used for determination of ethanol in two different wine samples and results were in good agreement with those obtained by gas chromatography.