Nanoparticle-Modified Electrochemical Immunosensor Platform for Cardiac Troponin Assay
19th International Nanoscience and Nanotechnology Conference (NanoTR-19), Ankara, Türkiye, 27 - 29 Ağustos 2025, ss.129, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Ankara
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.129
- Ankara Hacı Bayram Veli Üniversitesi Adresli: Evet
Özet
One of the most deadly diseases that occur in the heart and blood vessels is cardiovascular diseases. Due to their lethality, early diagnosis is of great
importance. In the presence of any of these diseases, electrochemical methods stand out because of the rapid, easy, cheap and highly sensitive detection of
cardiac biomarkers. One of the most important gold biomarkers of cardiovascular diseases is troponin I [1,2]. In this study, metal-based nanoparticles were
used for the detection of cardiac troponin I to improve the electroanalytical performance of the sensing platform. Metallic nanoparticles are very useful
materials used in the construction of beneficial immunosensors with their adjustable shape and morphology, effective surface area and advanced catalytic
and chemical properties [3,4]. In this study, we synthesized sponge-like bimetallic-based nanoparticles consisting platinum (Pt) and nickel (Ni) to modify
screen-printed carbon electrode (SPCE). To ensure immobilization of the antibody onto this modified electrode, we incorporated gold nanoparticles into
the structure using electrodeposition method. Characterization studies of the so-formed electrode were carried out with electrochemical impedance
spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). The proposed
electrode showed promising results for troponin I biomarker in terms of good sensitivity.