Disposable biosensors based on platinum nanoparticle-modified screen-printed carbon electrodes for the determination of biogenic amines

DALKIRAN B., KAÇAR SELVİ C., Can E., Erden P. E., Kılıç E.

Monatshefte fur Chemie, cilt.151, sa.12, ss.1773-1783, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 151 Sayı: 12
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s00706-020-02707-1
  • Dergi Adı: Monatshefte fur Chemie
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1773-1783
  • Anahtar Kelimeler: Biogenic amines, Amperometric biosensor, Screen-printed electrode, Platinum nanoparticles, Diamine oxidase, Monoamine oxidase, AMPEROMETRIC BIOSENSOR, ELECTROCHEMICAL DETERMINATION, ENZYME SENSOR, FOOD, HISTAMINE, NANOTUBES, IMMOBILIZATION, COMPOSITE, TYRAMINE, OXIDASE
  • Ankara Hacı Bayram Veli Üniversitesi Adresli: Evet

Özet

© 2020, Springer-Verlag GmbH Austria, part of Springer Nature.This work presents the development of disposable biosensors used in the determination of biogenic amines. The biosensors were fabricated using diamine oxidase (DAOx) or monoamine oxidase (MAOx) enzyme- and platinum nanoparticle (PtNP)-modified screen-printed carbon electrodes (SPCE). The morphological and electrochemical properties of the biosensors were examined by scanning electron microscopy, energy-dispersive X-ray spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry measurements. Amperometric measurements indicated that the DAOx/PtNP/SPCE biosensor responded to histamine, putrescine, cadaverine, spermine, spermidine, β-phenylethylamine, tryptamine, and tyramine; however, its MAOx-based counterpart showed no response towards putrescine and cadaverine. A performance comparison of two biosensors indicated that the one based on DAOx had a linear concentration range from 5.3 × 10− 7 to 7.2 × 10− 5 M and the other based on MAOx from 3.9 × 10− 7 to 7.6 × 10− 5 M for tyramine. The sensitivities of the DAOx- and MAOx-based biosensors towards tyramine were 24.8 µA mM− 1 and 46.1 µA mM− 1, respectively. The proposed biosensors were tested for analysis of blue cheese sample spiked with known concentration of tyramine for the verification of biosensor applicability for real samples. Graphic abstract: [Figure not available: see fulltext.]