Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP)

BAYRAMOĞLU G., Şenkal B. F., YILMAZ M., Arica M. Y.

BIORESOURCE TECHNOLOGY, vol.102, no.21, pp.9833-9837, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 102 Issue: 21
  • Publication Date: 2011
  • Doi Number: 10.1016/j.biortech.2011.08.042
  • Journal Name: BIORESOURCE TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.9833-9837
  • Keywords: SI-ATRP, Beads, Immobilized enzyme, Papain, Protein hydrolysis, ADSORPTION, MICROSPHERES
  • Ankara Haci Bayram Veli University Affiliated: No

Abstract

Poly(hydroxyethyl methacrylate-ethylen glycol dimethacrylate), p(HEMA-EGDMA), beads were prepared by suspension polymerization, and were decorated with fibrous poly(glycidyl methacrylate), p(GMA), via surface initiated-atom transfer radical polymerization (SI-ATRP). The functional epoxy groups of the beads were used for covalent immobilization of papain. The average amount of immobilized enzyme was 18.7 mg/g beads. The immobilized enzyme was characterized by temperature, pH, operational and storage stability experiments. The maximum velocity of the free and immobilized enzymes (V(max)) and Michaelis-Menten constant (K(m)) values were determined as 10.7 and 8.3 U/mg proteins and 274 and 465 mu M, respectively. The immobilized papain was operated in a batch reactor, and it was very effective for hydrolysis of different proteins (i.e., casein and cytochrom c). (C) 2011 Elsevier Ltd. All rights reserved.