Akademik Veri Yönetim Sistemi
Acta Stomatologica Croatica, cilt.58, sa.1, ss.2-17, 2024 (ESCI)
Objective: This study aimed to evaluate the biological effects of “proanthocyanidin” (PA), and “nisin”(Ni), on dental pulp stem cells (DPSCs) and LPS-induced DPSCs as well as their antimicrobial effectsagainst S. aureus and E. coli. Materials and methods: After characterization of DPSCs, cytotoxicityof PA and Ni on DPSCs were evaluated using a water-soluble tetrazolium salt (WST-1). The cytokinesand chemokines released by DPSCs and the expression levels of IL-6, IL-8, and TNF alpha were detectedwith human Cytokine Array C5 and enzyme-linked immunosorbent assay (ELISA), respectively. Theantibacterial activities of PA and Ni were tested using the drop plate method. Results: PA at 75 μg/mlincreased cell viability, decreased TNF-α expression of DPSCs, did not show any cytotoxic effects onLPS-induced DPSCs, and also showed a tendency to decrease TNF-α expression. PA at 75 μg/ml exhibitedhigher expressions of TIMP-2, OPG, IL-7, and IL-8 in LPS-induced DPSCs compared to DPSCs.Ni at 100 μg/ml decreased TNF-α expression in DPSCs with no cytotoxic effects. It provided increasedcell viability and a downregulation trend of TNF-α expression in LPS-induced DPSCs. Both Ni and PAprovided strong antibacterial effects against S. aureus. Ni at 200μg/ml had strong antibacterial effectsagainst E. coli without affecting negatively the viability of both DPSCs and LPS-induced DPSCsand showed anti-inflammatory activity by decreasing TNF-α expression. PA provided strong antibacterialeffects against E. coli at 200 μg/ml but affected DPSCs viability negatively. Conclusion: PA andNi at specific concentrations exhibited immunomodulatory activity on DPSCs and LPS-induced DPSCswithout any cytotoxic effects and strong antibacterial effects on S. aureus.