Amperometric Biosensors for Tyramine Determination Based on Graphene Oxide and Polyvinylferrocene Modified Screen-printed Electrodes

Erden P. E., Erdoğan Z. Ö., Öztürk F., Koçoğlu İ. O., Kılıç E.

Electroanalysis, cilt.31, sa.12, ss.2368-2378, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 12
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1002/elan.201900369
  • Dergi Adı: Electroanalysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2368-2378
  • Anahtar Kelimeler: Amperometric biosensor, biogenic amine, tyramine, polyvinlyferrocene, graphene oxide, GLASSY-CARBON ELECTRODE, BIOGENIC-AMINES, SELECTIVE DETERMINATION, ELECTROCHEMICAL SENSOR, GLUCOSE-OXIDASE, DIAMINE OXIDASE, IMMOBILIZATION, ACID, FILM, NANOPARTICLES
  • Ankara Hacı Bayram Veli Üniversitesi Adresli: Evet

Özet

© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, WeinheimA comparison of the analytical characteristics of two tyramine biosensors, based on graphene oxide (GRO) and polyvinylferrocene (PVF) modified screen-printed carbon electrodes (SPCE), is reported. Diamine oxidase (DAOx) or monoamine oxidase (MAOx) was immobilized onto the PVF/GRO modified SPCE to fabricate the biosensors. Surface characteristics and electrochemical behaviour of the modified SPCEs were investigated by atomic force microscopy (AFM), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX) and cyclic voltammetry (CV). Electrode surface composition and experimental variables such as pH and working potential were optimized in order to ensure a high performance. Under optimum experimental conditions, both DAOx/PVF/GRO/SPCE and MAOx/PVF/GRO/SPCE biosensors exhibited wide linear dynamic ranges for tyramine from 9.9×10−7 to 1.2×10−4 M and from 9.9×10−7 to 1.1×10−4 M, respectively. MAOx/PVF/GRO/SPCE biosensor showed higher sensitivity (11.98 μA mM−1) for tyramine determination than the DAOx/PVF/GRO/SPCE biosensor (7.99 μA mM−1). The substrate specifity of the biosensors to other biogenic amines namely histamine, putrescine, spermine, spermidine, tryptamine, β-phenylethylamine and cadaverine was also investigated. The developed biosensors were successfully used for tyramine determination in cheese sample.