Immobilization of catalase via adsorption on poly(styrene-d-glycidylmethacrylate) grafted and tetraethyldiethylenetriamine ligand attached microbeads

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BAYRAMOĞLU G., Karagöz B., YILMAZ M., Bicak N., Arica M. Y.

BIORESOURCE TECHNOLOGY, vol.102, no.4, pp.3653-3661, 2011 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 102 Issue: 4
• Publication Date: 2011
• Doi Number: 10.1016/j.biortech.2010.12.029
• Journal Name: BIORESOURCE TECHNOLOGY
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Page Numbers: pp.3653-3661
• Keywords: Catalase, Enzyme immobilization, Adsorption, Multi-modal ligand, Kinetics, TRANSFER RADICAL POLYMERIZATION, REVERSIBLE IMMOBILIZATION, BEADS, MEMBRANES, BRUSHES, ENZYMES, LACCASE, STABILIZATION, MICROSPHERES, PURIFICATION
• Ankara Haci Bayram Veli University Affiliated: No

Abstract

Fibrous poly(styrene-d-glycidylmethacrylate) (P(S-GMA)) brushes were grafted on poly(styrene-divinylbenzene) (P(S-DVB)) beads using surface initiated-atom transfer radical polymerization (SI-ATRP). Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The multi-modal ligand attached beads were used for reversible immobilization of catalase. The influences of pH, ionic strength and initial catalase concentration on the immobilization capacities of the P(S-DVB)-g-P(S-GMA)-TEDETA beads have been investigated. Catalase adsorption capacity of P(S-DVB-g-P(S-GMA)-TEDETA beads was found to be 40.8 +/- 1.7 mg/g beads at pH 6.5 (with an initial catalase concentration 1.0 mg/mL). The K-m value for immobilized catalase on the P(S-DVB-g-P(S-GMA)-TEDETA beads (0.43 +/- 0.02 mM) was found about 1.7-fold higher than that of free enzyme (0.25 +/- 0.03 mM). Optimum operational temperature and pH was increased upon immobilization. The same support was repeatedly used five times for immobilization of catalase after regeneration without significant loss in adsorption capacity or enzyme activity. (C) 2010 Elsevier Ltd. All rights reserved.