Molecular Determination of clfA Gene by Pcr and Dna Sequencing Technique

Radhi M. S. T. A., Dölarslan M., Mutur A. D.

Biochemical and Cellular Archives, vol.21, no.1, pp.63-68, 2021 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 2021
  • Journal Name: Biochemical and Cellular Archives
  • Journal Indexes: Scopus, BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database
  • Page Numbers: pp.63-68
  • Keywords: clfA gene, DND sequencing, S. aureus
  • Ankara Haci Bayram Veli University Affiliated: No


© 2021. All Rights Reserved.The pathogenic bacteria ( S. aureus ) is one of the principal imperative bacteria, that likewise has danger role in public health and can cause outbreaks in hospitals such as burn patients. The aim of this study is to prevalence of clfA gene by PCR technique and to identify the genetic polymorphisms of clumping factor A gene in S. aureus. Thirty five (87.5%) isolates were identified as S. aureus from 40 burn samples. The molecular results showed that the clfA was found only in 29 (82.86%). The current results indicated the absence of any detectable nucleic acid variations in the amplified 288bp fragment of the clfA gene. The generated clfR-based phylogenetic tree indicated accurate positioning with regard to the investigated samples. These comprehensive trees were constructed to allocate the identified mutations with their related organisms. The currently constructed phylogenetic provided informative comprehensive data, in terms of the accurate positioning of S. aureus samples and their relative sequences. Therefore, the generated clfA-based phylogenetic trees provided a confirmed identity with respect to the phylogenetic relationships among the investigated samples.