Amperometric enzyme electrodes for xanthine determination with different mediators


Erden P. E. , Pekyardimci Ş., Kiliç E.

Acta Chimica Slovenica, vol.59, no.4, pp.824-832, 2012 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 59 Issue: 4
  • Publication Date: 2012
  • Journal Name: Acta Chimica Slovenica
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.824-832
  • Keywords: 1,4-benzoquinone, Amperometry, Enzyme electrode, Poly(vinylferrocene), Xanthine, Xanthine oxidase
  • Ankara Haci Bayram Veli University Affiliated: No

Abstract

Two new amperometric carbon paste enzyme electrodes were developed for xanthine determination. 1,4-benzoquinone and poly(vinylferrocene) (PVF) were investigated as mediators. The parameters affecting the analytical performance of the enzyme electrode have been investigated in detail and optimized for modified enzyme electrodes. 1,4-benzoquinone modified enzyme electrode (BQ-CPEE) exhibited linear response from 1.9 × 10-7 M to 5.5 × 10-6 M and from 5.2 × 10-5 M to 8.2 × 10 -4 M with a good detection limit of 1.0 × 10-7 M. The linear working range of the PVF modified enzyme electrode was between 1.9 × 10-7-2.1 × 10-6 M, 1.9 × 10 -6-1.0 × 10-5 M and 1.1 × 10-4-8.8 × 10-4 M with a detection limit of 1.0 × 10-7 M. Hypoxanthine response of the electrodes was also determined. Modified enzyme electrodes were used for xanthine determination in real samples and good recoveries were obtained.