Akademik Veri Yönetim Sistemi
Biotechnology and Biotechnological Equipment, cilt.23, sa.3, ss.1299-1302, 2009 (SCI-Expanded)
Microsatellite markers are powerful tools for many evolutionary and genetic studies. They provide high level of polymorphism and information in cereal genomic research. However, development of microsatellite markers is extremely expensive and both time and labor consuming because of the requirement of prior sequence knowledge for design of locus specific primers. Therefore, use of microsatellite markers developed for one species could be very valuable for related species. In the present study we investigated the possibility of SSR marker transferability among cool season cereals. Transferability of 27 wheat and 23 barley microsatellites were studied using five wheat, five barley, two oat and two rye cultivars. Transferability of the barley SSR markers to wheat was 69.6%(16/23), to oat 43.5%(10/23) and to rye 52.2%(12/23). Of 27 wheat SSR markers 20(74%) were amplified in barley, 20(74%) in oat and 19(70.4%) in rye. These results show that about two or three out of every four microsatellites can be used in related cereal genera. © 2009 Taylor and Francis Group, LLC.