DNA adsorption on poly(N,N-dimethylacrylamide)-grafted chitosan hydrogels


Baser B., Demirel G., ÇAYKARA T.

Journal of Applied Polymer Science, vol.120, no.3, pp.1420-1425, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 120 Issue: 3
  • Publication Date: 2011
  • Doi Number: 10.1002/app.33029
  • Journal Name: Journal of Applied Polymer Science
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1420-1425
  • Keywords: hydrogels, N,N-dimethylacrylamide, chitosan, DNA adsorption, IN-VITRO RELEASE, ALGINATE
  • Ankara Haci Bayram Veli University Affiliated: No

Abstract

In this study, poly(N,N-dimethylacrylamide) grafted chitosan (PDMAAm-g-CT) hydrogels were prepared for deoxyribonucleic acid (DNA) adsorption. Instead of directly grafting the N,N-dimethylacrylamide (DMAAm) monomer onto the chitosan (CT) chains, poly(N,N-dimethylacrylamide) with carboxylic acid end group (PDMAAm-COOH) was firstly synthesized by free-radical polymerization using mercaptoacetic acid (MAAc) as the chain-transfer agent and then grafted onto the CT having amino groups. The synthesis of PDMAAm-COOH and its grafting onto the CT chains were confirmed by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. From gel permeation chromatography measurements, the number-average molecular weight (Mn) and polydispersity index of PDMAAm-COOH were found as 2400 g/mol and 2.3, respectively. The PDMAAm-g-CT hydrogels were utilized as the adsorbents in DNA adsorption experiments conducted at +4°C in a trisEDTA solution of pH 7.4. The hydrogels produced with higher PDMAAm-COOH content exhibited higher DNA adsorption capacity. The DNA adsorption capacity up to 4620 μg DNA/g dry gel could be achieved with the PDMAAm-g-CT hydrogels prepared in 80.0 wt % PDMAAm-COOH feed concentration. This value is approximately seven times higher than that of CT alone. © 2010 Wiley Periodicals, Inc.