An amperometric hydrogen peroxide biosensor based on Co3O4 nanoparticles and multiwalled carbon nanotube modified glassy carbon electrode

KAÇAR SELVİ C., DALKIRAN B., ERDEN P. E., Kilic E.

APPLIED SURFACE SCIENCE, vol.311, pp.139-146, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 311
  • Publication Date: 2014
  • Doi Number: 10.1016/j.apsusc.2014.05.028
  • Journal Name: APPLIED SURFACE SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.139-146
  • Keywords: Hydrogen peroxide, Amperometry, Horseradish peroxidase, Co3O4 nanoparticle, Multiwall carbon nanotube, Gelatin, MODIFIED GOLD ELECTRODE, HORSERADISH-PEROXIDASE, GLUCOSE BIOSENSOR, COMPOSITE FILM, ELECTROCHEMICAL DETECTION, XANTHINE-OXIDASE, HYBRID MATERIALS, FISH MEAT, H2O2, IMMOBILIZATION
  • Ankara Haci Bayram Veli University Affiliated: Yes

Abstract

In this work a new type of hydrogen peroxide biosensor was fabricated based on the immobilization of horseradish peroxidase (HRP) by cross-linking on a glassy carbon electrode (GCE) modified with Co3O4 nanoparticles, multiwall carbon nanotubes (MWCNTs) and gelatin. The introduction of MWCNTs and Co3O4 nanoparticles not only enhanced the surface area of the modified electrode for enzyme immobilization but also facilitated the electron transfer rate, resulting in a high sensitivity of the biosensor. The fabrication process of the sensing surface was characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Amperometric detection of hydrogen peroxide was investigated by holding the modified electrode at -0.30 V (vs. Ag/AgCl). The biosensor showed optimum response within 5 s at pH 7.0. The optimized biosensor showed linear response range of 7.4 x 10(-7)-1.9 x 10(-5) M with a detection limit of 7.4 x 10(-7). The applicability of the purposed biosensor was tested by detecting hydrogen peroxide in disinfector samples. The average recovery was calculated as 100.78 +/- 0.89. (C) 2014 Elsevier B.V. All rights reserved.