Long-Term Storage and Reusability of Living Bacteria-Encapsulated Polyethylene Oxide Nanofiber Mats for Reactive Dye Removal
Journal of Nano Research, cilt.84, ss.83-94, 2024 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 84
- Basım Tarihi: 2024
- Doi Numarası: 10.4028/p-7wo98o
- Dergi Adı: Journal of Nano Research
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
- Sayfa Sayıları: ss.83-94
- Anahtar Kelimeler: Bioremediation, Dye, Electrospinning, Nanofiber, Viability
- Ankara Hacı Bayram Veli Üniversitesi Adresli: Evet
Özet
From food to environmental applications, the encapsulation of bio-objects in nanofibers is widely used. In particular, for improved and sustainable performance of bioremediation, the system requires the development of cost-effective nanomaterials that containing living microorganisms for textile wastewater treatment is required. Here, bacteria-encapsulated polyethylene oxide (PEO) nanofibers (Nfs) were prepared by electrospinning. According to the Scanning Electron Microscope (SEM) images, PEO-Nfs show bead-free morphology and homogeneous distribution, while random expansions are observed in the nanofibers after bacterial encapsulation. While the number of live bacteria in the polymer before electrospinning was 1010 CFU/mL, the number of live bacteria-encapsulated after spinning was 108 CFU/mL. This proves that nanofibers carry a very high number of bacteria after electrospinning which is supported by Fluorescence microscope images. Furthermore, an ATR-FTIR study confirmed the molecular interactions between PEO and bacteria in the nanofibers. The removal efficiency of PEO_bacteria-Nfs was 26.6 ± 0.3% at 5 ppm and 9 ± 0.1% at 20 ppm dye concentration. Under storage conditions of +4 °C, the bacteria-encapsulated in PEO-Nfs show cell viability for more than 60 days. In order to extend the research on bacteria-encapsulated polymer Nfs, we explored the possibility of extending the life of bacteria in electrospun Nfs by cross-linking approaches using non-toxic calcium ions. The composite Nf mats were therefore reused for up to 4 repeated cycles.