Disposable biosensor based on nanodiamond particles, ionic liquid and poly-L-lysine for determination of phenolic compounds

Şener D., Erden P. E., KAÇAR SELVİ C.

Analytical Biochemistry, cilt.688, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 688
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1016/j.ab.2024.115464
  • Dergi Adı: Analytical Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, Veterinary Science Database
  • Anahtar Kelimeler: Amperometric biosensor, Catechol, Ionic liquid, Nanodiamond, Phenolic compound, Poly-L-lysine
  • Ankara Hacı Bayram Veli Üniversitesi Adresli: Evet

Özet

This study describes the development of a highly sensitive amperometric biosensor for the analysis of phenolic compounds such as catechol. The biosensor architecture is based on the immobilization of tyrosinase (Tyr) on a screen-printed carbon electrode (SPE) modified with nanodiamond particles (ND), 1-butyl-3-methylimidazolium hexafluorophosphate (IL) and poly-L-lysine (PLL). Surface morphologies of the electrodes during the modification process were evaluated by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to investigate the electrochemical characteristics of the modified electrodes. Owing to the synergistic effect of the modification materials, the Tyr/PLL/ND−IL/SPE exhibited high sensitivity (328.2 μA mM−1) towards catechol with a wide linear range (5.0 × 10−8 – 1.2 × 10−5 M) and low detection limit (1.1 × 10−8 M). Furthermore, the method demonstrated good reproducibility and stability. The amperometric response of the biosensor towards other phenolic compounds such as bisphenol A, phenol, p-nitrophenol, m-cresol, p-cresol and o-cresol was also investigated. The analytical applicability of the biosensor was tested by the analysis of catechol in tap water. The results of the tap water analysis showed that the Tyr/PLL/ND−IL/SPE can be used as a practical and effective method for catechol determination.