Amperometric biogenic amine biosensors based on Prussian blue, indium tin oxide nanoparticles and diamine oxidase– or monoamine oxidase–modified electrodes

KAÇAR SELVİ C., Erden P. E., DALKIRAN B., İNAL E. K., Kiliç E.

Analytical and Bioanalytical Chemistry, cilt.412, sa.8, ss.1933-1946, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 412 Sayı: 8
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s00216-020-02448-4
  • Dergi Adı: Analytical and Bioanalytical Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, DIALNET, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.1933-1946
  • Anahtar Kelimeler: Amperometry, Biosensor, Biogenic amine, Indium tin oxide, Prussian blue, Screen-printed electrode, SCREEN-PRINTED ELECTRODES, ELECTROCHEMICAL BIOSENSOR, HYDROGEN-PEROXIDE, DISPOSABLE BIOSENSOR, GLUCOSE-OXIDASE, SENSOR, LAYER, GOLD, IMMOBILIZATION, NANOCOMPOSITE
  • Ankara Hacı Bayram Veli Üniversitesi Adresli: Evet

Özet

© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.Biogenic amine biosensors, based on screen-printed carbon electrodes (SPCE) modified with Prussian blue (PB) and indium tin oxide nanoparticles (ITONP), are reported. PB/ITONP-modified SPCE was further modified with diamine oxidase (DAO) or monoamine oxidase (MAO) enzymes to construct the biosensors. The morphology of the modified electrodes was studied by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to enlighten the electrochemical properties of the modified electrodes at each step of biosensor fabrication. Electrode surface composition and experimental conditions were optimized and analytical performance characteristics of the biosensors were studied. Several biogenic amines were tested and both biosensors responded to histamine, putrescine and cadaverine. DAO/ITONP/PB/SPCE biosensor exhibited the highest response to histamine 6.0 × 10−6–6.9 × 10−4 M with a sensitivity of 1.84 μA mM−1. On the other hand, the highest sensitivity was obtained for cadaverine with the MAO/ITONP/PB/SPCE biosensor. The analytical utility of the presented biosensors were illustrated by the determination of cadaverine and histamine in cheese sample.