An amperometric enzyme electrode for creatine determination prepared by the immobilization of creatinase and sarcosine oxidase in poly(vinylferrocenium)

ERDEN P. E. , Pekyardimci S., Kilic E., Arslan F.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.34, no.2, pp.223-239, 2006 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 2
  • Publication Date: 2006
  • Doi Number: 10.1080/10731190600581775
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.223-239
  • Keywords: creatine, enzyme electrode, amperometry, poly(vinylferrocen), creatinase, sarcosine oxidase, GLUCOSE-OXIDASE, PROTEIN, ASSAY, SERUM, ACID, FILM


A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and bovine serum albumin (BSA) was selected as the best immobilization method for the enzymatic system. Determination of creatine was performed by the oxidation of enzymatically generated H2O2 at +0.7 V vs. Ag/AgCl. The linear working range of the electrode was 2.0 x 10(-5) -3.2 x 10(-4) M and the response time was about 50 s. The effects of pH, temperature, enzyme ratio and buffer concentration were investigated and optimum parameters were found to be 7.5, 37 degrees C, 2.5:1 (CI:SO) and 0.05 M, respectively. The stability and reproducibility of the enzyme electrode have been also studied.