© 2022Development of novel sensing and actuating systems that can mimic the properties of natural materials has been one of the milestones in the biomedical field. By this opportunity, in the current study, tungsten disulfide (WS2) incorporated poly(3,4-ethylenedioxythiophene:polystyrene sulfonate) (WS2-PEDOT:PSS) modified electrode was created using effortless and straightforward electrochemical procedure. Scanning electron microscopy (SEM), X-ray energy dispersive analysis (EDX) and X-ray diffraction (XRD) analysis were used to identify the structural composition, particularly tungsten (W) presence. Thereafter the designed surface was applied for two different approaches. In both approaches, transition metal dichalcogenide combination into the polymeric structure was evaluated in terms of improved surface properties such as high active surface area and sensitivity. Firstly, WS2-PEDOT:PSS modified pencil graphite electrode (PGE) was used as a sensitive Epirubicin (EPI) sensor. The electrode responded to EPI in a linear concentration range of 0.1 mg L−1 to 200 mg L−1 (0.172 μM to 345 μM) with a detection limit of 0.02 mg L−1 (35 nM) (n = 3). Secondly, EPI was encapsulated into the WS2-PEDOT:PSS during polymerization step and actuating property of the so-formed electrode (WS2-PEDOT:PSS/EPI/PGE) was investigated by electrical and pH stimuli. It was observed that the electrical stimuli showed a good actuator profile when a potential of -0.5 V was set. In addition, this EPI loaded electrode was characterized under a fluorescence microscope before/after stimuli.