Amperometric uric acid biosensor based on poly(vinylferrocene)-gelatin-carboxylated multiwalled carbon nanotube modified glassy carbon electrode


ERDEN P. E. , KAÇAR SELVİ C., ÖZTÜRK F., Kilic E.

TALANTA, vol.134, pp.488-495, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 134
  • Publication Date: 2015
  • Doi Number: 10.1016/j.talanta.2014.11.058
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.488-495
  • Keywords: Amperometry, Uric acid, Biosensor, Poly(vinylferrocene), Mediator, Carboxylated multiwalled carbon nanotube, ENZYME ELECTRODE, POLY(VINYLFERROCENIUM) FILM, POLYPYRROLE FILM, COMPOSITE FILM, IMMOBILIZATION, SERUM, OXIDASE, SENSOR, GLUCOSE, CONSTRUCTION
  • Ankara Haci Bayram Veli University Affiliated: Yes

Abstract

In this study, a new uric acid biosensor was constructed based on ferrocene containing polymer poly (vinylferrocene) (PVF), carboxylated multiwalled carbon nanotubes (c-MWCNT) and gelatin (GEL) modified glassy carbon electrode (GCE). Uricase enzyme (UOx) was immobilized covalently through N-ethyl-N'-(3-dimethyaminopropyl) carbodiimide (EDC) and N-hydroxyl succinimide (NHS) chemistry onto c-MWCNT/GEL/PVF/GCE. The c-MWCNT/GEL/PVF composite was characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. Various experimental parameters such as pH, applied potential, enzyme loading, PVF and c-MWCNT concentration were investigated in detail. Under the optimal conditions the dynamic linear range of uric acid was 2.0 x 10(-7) M - 7.1 x 10(-4) M (R=0.9993) with the detection limit low to 2.3 x 10(-8) M. With good selectivity and sensitivity, the biosensor was successfully applied to determine the uric acid in human serum. The results of the biosensor were in good agreement with those obtained from standard method. Therefore, the presented biosensor could be a good promise for practical applications in real samples. (C) 2014 Elsevier B.V. All rights reserved.