Amperometric Lactate Biosensor Based on Carbon Paste Electrode Modified with Benzo[c]cinnoline and Multiwalled Carbon Nanotubes

Celik A. C., ÖZTÜRK F., ERDEN P. E., KAÇAR SELVİ C., Kilic E.

ELECTROANALYSIS, vol.27, no.12, pp.2820-2828, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 12
  • Publication Date: 2015
  • Doi Number: 10.1002/elan.201500180
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2820-2828
  • Keywords: Amperometry, benzo[c]cinnoline, L-lactate, redox mediator, multiwalled carbon nanotubes, L-LACTIC ACID, ELECTROCHEMICAL BIOSENSOR, FILM ELECTRODES, OXIDASE, COMPOSITE, GLUCOSE, IMMOBILIZATION, DEHYDROGENASE, GRAPHENE, BEHAVIOR
  • Ankara Haci Bayram Veli University Affiliated: Yes

Abstract

Amperometric lactate biosensor based on a carbon paste electrode modified with benzo[c]cinnoline and multiwalled carbon nanotubes is reported. Incorporation of benzo[c] cinnoline acting as a mediator and multiwalled carbon nanotubes providing a conduction pathway to accelerate electron transfer due to their excellent conductivity into carbon paste matrix resulted in a high performance lactate biosensor. The resulting biosensor exhibited a fast response, high selectivity, good repeatability and storage stability. Under the optimal conditions, the enzyme electrode showed the detection limit of 7.0 x 10(-8) M with a linear range of 2.0 x 10(-7) M-1.1 x 10(-4) M. The usefulness of the biosensor was demonstrated in serum samples.