© 2021 SAGE Publications.The aim of this study is to examine the moderating effects of gender relationship between quality of work life and job satisfaction using total quality management perceptions as a mediating factor. Data were collected from Private and Ministry of Health Training and Research Hospitals using 515 employees in Turkey. The moderated mediation analysis was set up according to the hypothesis model. Total quality management is the mediator, and gender is the moderator. The findings showed that the relationship between quality of work life and job satisfaction, quality of work life and total quality management, total quality management and job satisfaction. With respect to the gender variable, the total quality of management decreases; job satisfaction is a little stronger in males. However, even if job satisfaction increases, it is equal in both males and females.